2WK7

Structure of apo form of Vibrio cholerae CqsA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	P 21 21 2
Unit cell lengths	177.314, 70.077, 70.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.639 - 2.500
R-factor	0.2104
Rwork	0.206
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.120
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	177.000	2.530
High resolution limit [Å]	2.500	2.500
Rmerge	0.070	0.550
Number of reflections	29524
<I/σ(I)>	25	3
Completeness [%]	94.0	88
Redundancy	2.9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1