2WJX
Crystal structure of the human ionotropic glutamate receptor GluR2 ATD region at 4.1 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 224.253, 224.253, 76.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.379 - 4.100 |
| R-factor | 0.2928 |
| Rwork | 0.290 |
| R-free | 0.35390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.120 | 0.840 |
| Number of reflections | 15949 | |
| <I/σ(I)> | 23.3 | 5.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 28.5 | 29.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M BIS-TRIS PROPANE, PH 7.5 0.2M POTASSIUM/SODIUM TARTRATE 20% (W/V) PEG3350 |
