2WJL
Bacteriorhodopsin mutant E194D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.220, 61.220, 111.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.015 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.330 |
| Number of reflections | 12843 | |
| <I/σ(I)> | 22.18 | 4.97 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 6.2965 | 6.16502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | LIPID CUBIC PHASE AFTER LUECKE ET AL., pH 5.6 |
