2WJL
Bacteriorhodopsin mutant E194D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 63 |
Unit cell lengths | 61.220, 61.220, 111.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.007 |
RMSD bond angle | 0.015 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.070 | 0.330 |
Number of reflections | 12843 | |
<I/σ(I)> | 22.18 | 4.97 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 6.2965 | 6.16502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | LIPID CUBIC PHASE AFTER LUECKE ET AL., pH 5.6 |