2WJK

Bacteriorhodopsin mutant E204D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	60.810, 60.810, 110.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3w
RMSD bond length	0.008
RMSD bond angle	0.014
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.340
Number of reflections	10188
<I/σ(I)>	18.12	4.97
Completeness [%]	98.5	100
Redundancy	5.06203	5.0169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6		LIPID CUBIC PHASE AFTER LUECKE ET AL., pH 5.6