2WJJ

Structure and function of the FeoB G-domain from Methanococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-25
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	84.670, 84.670, 137.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.202 - 2.405
R-factor	0.1848
Rwork	0.183
R-free	0.22790
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.136
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.070	1.130
Number of reflections	37096
<I/σ(I)>	18	1.92
Completeness [%]	99.0	91.9
Redundancy	9.7	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1