2WJH

Structure and function of the FeoB G-domain from Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-08
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	104.200, 50.400, 94.700
Unit cell angles	90.00, 120.60, 90.00

Refinement procedure

Resolution	42.944 - 2.100
R-factor	0.2122
Rwork	0.210
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wjg
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.550
Number of reflections	24931
<I/σ(I)>	14.5	2.9
Completeness [%]	99.6	99.7
Redundancy	10.2	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1