2WJE
Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.980, 58.540, 114.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.987 - 1.899 |
| R-factor | 0.1412 |
| Rwork | 0.139 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNRELEASED STRUCTURE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.020 | 0.040 |
| Number of reflections | 22224 | |
| <I/σ(I)> | 64.4 | 27.8 |
| Completeness [%] | 92.5 | 61.4 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-ACETATE, 35% W/V PEG 400, PH 4.2 |
