2WIZ
Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 122.650, 122.650, 125.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.560 - 3.300 |
| R-factor | 0.2079 |
| Rwork | 0.204 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wcz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.120 | 1.030 |
| Number of reflections | 8420 | |
| <I/σ(I)> | 16.18 | 2.69 |
| Completeness [%] | 95.0 | 86.5 |
| Redundancy | 9.34 | 9.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% W/V PEG 3350, 0.2 M AMMONIUM ACETATE, pH 7.5 |
