2WIM
Crystal structure of NCAM2 IG1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.390, 106.780, 188.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.574 - 3.000 |
| R-factor | 0.2171 |
| Rwork | 0.213 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.570 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.390 |
| Number of reflections | 15703 | |
| <I/σ(I)> | 16.6 | 3.8 |
| Completeness [%] | 91.9 | 96.1 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 14 % PEG-3350, 20 MM CACL2, 0.1 M CITRIC ACID PH 4.5 |
