2WIE

High-resolution structure of the rotor ring from a proton dependent ATP synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-01
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	93.360, 93.360, 257.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.931 - 2.130
R-factor	0.1984
Rwork	0.197
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yce
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	20.000
High resolution limit [Å]	2.130	2.130
Rmerge	0.150	0.990
Number of reflections	37848
<I/σ(I)>	9.6	1.7
Completeness [%]	99.2	96.7
Redundancy	4.7	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1