2WIA
Crystal Structures of the N-terminal Intracellular Domain of FeoB from Klebsiella Pneumoniae in Apo Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.204, 84.204, 122.457 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.130 - 2.450 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF FEOB IN GMPPNP STATE |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.700 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.130 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.100 | 0.280 |
| Number of reflections | 18109 | |
| <I/σ(I)> | 18.9 | 6.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS-HCL PH 8.5, 30% PEG4000, 200 MM MGCL2 |
