2WHV
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 (ALL CATION BINDING SITES OCCUPIED BY CALCIUM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 151.290, 151.290, 136.881 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.250 - 2.360 |
| R-factor | 0.20072 |
| Rwork | 0.200 |
| R-free | 0.22139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wcp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.143 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.250 | 2.400 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 24907 | |
| <I/σ(I)> | 17.65 | 2.9 |
| Completeness [%] | 99.7 | 97.1 |
| Redundancy | 6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 1.1 M CALCIUM CHLORIDE DIHYDRATE, 0.15 M POTASSIUM CHLORIDE |
