2WHU
Fluorescent Protein mKeima at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-06 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.110, 57.750, 93.070 |
| Unit cell angles | 90.00, 108.99, 90.00 |
Refinement procedure
| Resolution | 44.810 - 2.650 |
| R-factor | 0.23892 |
| Rwork | 0.235 |
| R-free | 0.30694 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wht |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.174 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 33282 | |
| <I/σ(I)> | 13.6 | 3.7 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 21% PEG 6000, 0.4M NACL, 0.15M PHOSPHATE PH5.6, VAPOR DIFFUSION, TEMPERATURE 293K, pH 8 |
