2WHT
Fluorescent Protein mKeima at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.457, 55.565, 90.562 |
| Unit cell angles | 90.00, 108.47, 90.00 |
Refinement procedure
| Resolution | 45.130 - 1.900 |
| R-factor | 0.18544 |
| Rwork | 0.183 |
| R-free | 0.23886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p4m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.827 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 0.650 |
| Number of reflections | 80367 | |
| <I/σ(I)> | 11.8 | 4 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 21% PEG 6000, 0.4M NACL, 0.15M SODIUM PHOSPHATE PH5.6, VAPOR DIFFUSION, TEMPERATURE 293K |
