2WHS
Fluorescent Protein mKeima at pH 3.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.570, 98.100, 123.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.700 - 2.100 |
| R-factor | 0.19302 |
| Rwork | 0.190 |
| R-free | 0.24788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wht |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.866 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 65627 | |
| <I/σ(I)> | 11.3 | 4.2 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 3.8 | 293 | 0.2M LITHIUM SULPHATE, 20% PEG 3350, 0.1M PHOSPHATE CITRATE PH 3.8, VAPOR DIFFUSION, TEMPERATURE 293K |
