2WHJ
Understanding how diverse mannanases recognise heterogeneous substrates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 111.970, 111.970, 47.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.350 - 1.780 |
| R-factor | 0.122 |
| Rwork | 0.119 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bqc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.050 | 0.100 |
| Number of reflections | 32878 | |
| <I/σ(I)> | 42 | 6.8 |
| Completeness [%] | 94.0 | 45 |
| Redundancy | 4.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8M AMSO4, 0.1M NA-CITRATE PH 6.5 |
