2WH7

The partial structure of a group A streptpcoccal phage-encoded tail fibre hyaluronate lyase Hylp2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	50.838, 50.838, 219.031
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	109.760 - 1.600
R-factor	0.214
Rwork	0.212
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c3f
RMSD bond length	0.020
RMSD bond angle	1.589
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0082)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.070	0.170
Number of reflections	23240
<I/σ(I)>	41.9	13
Completeness [%]	91.0	93
Redundancy	4.4	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2 M NACL, 10 % (W/V) PEG 6000;