2WGZ
Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.146, 94.222, 94.465 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 25.290 - 2.120 |
| R-factor | 0.16959 |
| Rwork | 0.169 |
| R-free | 0.20582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.090 | 0.220 |
| Number of reflections | 44578 | |
| <I/σ(I)> | 12.4 | 5.14 |
| Completeness [%] | 94.4 | 80.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 5% PEG 6000, 0.1M TRIS-HCL, PH 8.0 IN THE PRESENCE OF 10MM MNCL2 AND 10MM UDP |
