2WGV
Crystal structure of the OXA-10 V117T mutant at pH 6.5 inhibited by a chloride ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.750, 101.700, 126.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.190 - 1.800 |
| R-factor | 0.18354 |
| Rwork | 0.182 |
| R-free | 0.20909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4f |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.262 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.190 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 1.240 |
| Number of reflections | 59000 | |
| <I/σ(I)> | 15.2 | 1.6 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.8 M AS BUFFER, CITRATE 0.1 M PH 6.5 |
