2WGM
Complete ion-coordination structure in the rotor ring of Na-dependent F-ATP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 146.690, 139.280, 151.910 |
| Unit cell angles | 90.00, 118.40, 90.00 |
Refinement procedure
| Resolution | 39.678 - 2.350 |
| R-factor | 0.2215 |
| Rwork | 0.220 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yce |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.180 | 1.180 |
| Number of reflections | 220545 | |
| <I/σ(I)> | 8.8 | 1.8 |
| Completeness [%] | 98.7 | 91 |
| Redundancy | 11 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
