2WGD
Crystal structure of KasA of Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector | RIGAKU IMAGING PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.529, 77.529, 146.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.778 - 2.010 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gp6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.296 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.550 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 34607 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-PROPANOL, NACL HEPES PH 7.5 |
