2WG6
Proteasome-Activating Nucleotidase (PAN) N-domain (57-134) from Archaeoglobus fulgidus fused to GCN4, P61A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.350, 91.380, 103.360 |
| Unit cell angles | 90.00, 119.97, 90.00 |
Refinement procedure
| Resolution | 34.240 - 2.500 |
| R-factor | 0.19754 |
| Rwork | 0.196 |
| R-free | 0.22242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wg5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.240 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | 0.760 |
| Number of reflections | 57981 | |
| <I/σ(I)> | 16.91 | 2.03 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 4.25 | 4.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS PH 8.6, 1 M NH4H2PO4, 25% PEG 200 |
