2WFQ
Crystal structure of the N-terminal signalling domain of human Dhh without calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.563, 42.048, 84.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.18918 |
| Rwork | 0.187 |
| R-free | 0.22895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 12895 | |
| <I/σ(I)> | 14.6 | 4.5 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 12.7 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL, PH 8.5 0.2 M LITHIUM SULFATE 0.2 M SODIUM THIOCYANATE 30% (W/V) PEG4000 |
