2WFK

Calcium bound LipL32

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	287
Spacegroup name	P 43 21 2
Unit cell lengths	121.905, 121.905, 206.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.259 - 2.300
R-factor	0.221
Rwork	0.219
R-free	0.26180
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.903
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.420
Number of reflections	69717
<I/σ(I)>	47.2	6.7
Completeness [%]	99.7	100
Redundancy	13.8	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				TACSIMATE, PH7.0