2WFJ
Atomic resolution crystal structure of the PPIase domain of human cyclophilin G in complex with cyclosporin A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.319, 64.913, 69.285 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.750 |
| R-factor | 0.111 |
| R-free | 0.12900 |
| Structure solution method | DIRECT METHODS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.035 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 0.780 |
| High resolution limit [Å] | 0.750 | 0.750 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 212972 | |
| <I/σ(I)> | 11.6 | 3.63 |
| Completeness [%] | 99.1 | 90.4 |
| Redundancy | 5.25 | 3.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 28-32% (W/V) PEG 4000, 0.2M MGCL2, 0.1M TRIS PH 8.5 |
