2WFI
Atomic resolution crystal structure of the PPIase domain of human cyclophilin G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.544, 65.144, 69.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 0.750 |
R-factor | 0.107 |
R-free | 0.12400 |
Structure solution method | DIRECT METHODS |
Starting model (for MR) | NONE |
RMSD bond length | 0.020 |
RMSD bond angle | 0.039 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.760 |
High resolution limit [Å] | 0.750 | 0.750 |
Rmerge | 0.060 | 0.520 |
Number of reflections | 206194 | |
<I/σ(I)> | 17.4 | 1.68 |
Completeness [%] | 93.5 | 79.1 |
Redundancy | 7.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 28-32% PEG 4000 (W/V), 0.2M MGCL2, 0.1M TRIS PH 8.5 |