2WFD
Structure of the human cytosolic leucyl-tRNA synthetase editing domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.540, 94.540, 148.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.240 - 3.250 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD (SHARP) |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.000 | 3.250 |
| Rmerge | 0.150 | 0.660 |
| Number of reflections | 15008 | |
| <I/σ(I)> | 7.46 | 1.98 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 3.12 | 3.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 8.3, 20% W/V POLYETHYLENE GLYCOL 3350 |
