2WFC
Crystal structure of peroxiredoxin 5 from Arenicola Marina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.210, 112.070, 68.910 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 68.360 - 1.750 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hd2 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.170 | 1.790 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 51140 | |
| <I/σ(I)> | 17.7 | 4.4 |
| Completeness [%] | 95.6 | 82.5 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 4000 29%(W/V), DTT 0.001 M |
