2WFB
High resolution structure of the apo form of the orange protein (ORP) from Desulfovibrio gigas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.078, 43.078, 106.085 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 106.000 - 2.000 |
| R-factor | 0.18585 |
| Rwork | 0.183 |
| R-free | 0.24448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o13 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.650 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.090 |
| Number of reflections | 7446 | |
| <I/σ(I)> | 33.7 | 16.9 |
| Completeness [%] | 90.7 | 62.2 |
| Redundancy | 9.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.1 M NA ACETATE, 0.2 M NAH2PO4 PH 4.5, 15-30% PEG 3K OR PEG 8K |
