2WEX

Crystal structure of human apoM in complex with glycerol 1- myristic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX
Spacegroup name	P 43 21 2
Unit cell lengths	49.820, 49.820, 145.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 2.000
R-factor	0.21075
Rwork	0.208
R-free	0.25708
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wew
RMSD bond length	0.018
RMSD bond angle	1.800
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.050	0.540
Number of reflections	13097
<I/σ(I)>	23.53	4.11
Completeness [%]	99.7	99.7
Redundancy	8.41	8.49

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		ABOUT 1-2 MICROL OF PROTEIN (10-16 MG/ML IN 20 MM TRIS-HCL, PH 7.2) WERE MIXED WITH 1-2 MICROL OF 30 % PEG1500 (W/V, IN DEIONISED WATER) AND EQUILIBRATED AGAINST 700 MICROL OF RESERVOIR SOLUTION CONTAINING 30 % PEG1500.