2WEQ
Yeast Hsp90 N-terminal domain LI-IV mutant with Geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.608, 74.608, 110.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.780 - 2.200 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE HSP90 N-TERMINAL MODEL. |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.175 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 16617 | |
| <I/σ(I)> | 16.7 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
