2WEE
Crystal structure of Rv0371c from Mycobacterium tuberculosis H37Rv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.219, 61.019, 143.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.250 - 1.650 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2we9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.490 |
| Number of reflections | 40721 | |
| <I/σ(I)> | 37.6 | 3.36 |
| Completeness [%] | 89.4 | 98.3 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M MES PH 6.0 AND 18% PEG 4000 BY MICRO-SEEDING |
