2WE9
Crystal structure of Rv0371c from Mycobacterium tuberculosis H37Rv
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.181, 58.731, 143.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.670 - 2.100 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.28800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 20560 | |
| <I/σ(I)> | 41.96 | 5 |
| Completeness [%] | 95.4 | 73.6 |
| Redundancy | 11.9 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M MES PH 6.0 AND 18% PEG 4000 BY MICRO-SEEDING |
