2WDV
E. coli succinate:quinone oxidoreductase (SQR) with an empty quinone- binding pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 120.340, 184.847, 204.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.850 - 3.200 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23300 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.850 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.150 | 0.700 |
| Number of reflections | 76081 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS PH 8.5, 0.1M MGSO4, 10% PEG4000 |
