2WCS

Crystal Structure of Debranching enzyme from Nostoc punctiforme (NPDE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	95
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	93.731, 93.731, 257.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 2.800
R-factor	0.224
Rwork	0.224
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wc7
RMSD bond length	0.005
RMSD bond angle	1.100
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.560
Number of reflections	16274
<I/σ(I)>	11.3	7.2
Completeness [%]	100.0	100
Redundancy	22.8	23.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		20%(W/V)POLYETHYLENE GYLCOL, 200MM TRIC-CL (PH8.5),100MM MGCL2