2WCQ
Crystal Structure of Tip-Alpha N34 (HP0596) from Helicobacter pylori at pH4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.360, 101.990, 47.255 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 47.140 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.482 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.960 | 2.000 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 22965 | |
| <I/σ(I)> | 7.62 | 2.23 |
| Completeness [%] | 98.4 | 90.4 |
| Redundancy | 4.08 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 100 MM CITRATE PH 4, 18% PEG 4000, 15 MM ATP |
