2WCI
Structure of E. coli monothiol glutaredoxin GRX4 homodimer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97931, 1.73944, 1.74128 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.300, 94.300, 62.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.632 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 22331 | |
| <I/σ(I)> | 22.8 | 1.9 |
| Completeness [%] | 97.6 | 94.4 |
| Redundancy | 14.1 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25 % PEG 6000 0.1 M HEPES PH 7.5 |
