2WC9
Crystal structure of the g2p (large terminase) nuclease domain from the bacteriophage SPP1 with bound Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-20 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.710, 69.710, 73.801 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.600 - 2.500 |
| R-factor | 0.19781 |
| Rwork | 0.195 |
| R-free | 0.25862 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.217 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.400 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 7520 | |
| <I/σ(I)> | 32.4 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 80 MM TRIS PH 8.5, 2.3 M LI2SO4 |
