2WBZ
1.6 A Structure of Thaumatin Crystallized without Tartrate at 4 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.195, 58.195, 150.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.430 - 1.600 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0087) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 35012 | |
| <I/σ(I)> | 29.7 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 277 | PROTEIN AT 47 MG/ML IN 10MM SODIUM PHOSPHATE (PH=7.3) AND 5% (V/V) GLYCEROL; CRYSTALS GROWN AT 4 C. |
