2WBX

Crystal structure of mouse cadherin-23 EC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-08
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	45.891, 49.540, 45.883
Unit cell angles	90.00, 98.97, 90.00

Refinement procedure

Resolution	25.540 - 1.500
R-factor	0.17343
Rwork	0.172
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ff5
RMSD bond length	0.012
RMSD bond angle	1.368
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.050	0.490
Number of reflections	16290
<I/σ(I)>	18.7	2.5
Completeness [%]	99.8	99.8
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1 M SODIUM CACODYLATE PH 6.0 40% MPD