2WBT
The Structure of a Double C2H2 Zinc Finger Protein from a Hyperthermophilic Archaeal Virus in the Absence of DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2833, 1.2830, 1.1696 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.378, 120.464, 51.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.034 |
| RMSD bond angle | 1.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 8209 | |
| <I/σ(I)> | 17.2 | 4.4 |
| Completeness [%] | 78.7 | 78.8 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | SITTING DROP VAPOR DIFFUSION, 20 MG/ML OF B129 IN 20 MM BIS-TRIS, PH 6.5, 60 MM NACL MIXED WITH 0.1 M AMMONIUM CITRATE DIBASIC, 10-14 % PEG 4000 |
