2WBO
Crystal structure of VioC in complex with L-arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-07-15 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.630, 67.340, 62.420 |
Unit cell angles | 90.00, 108.83, 90.00 |
Refinement procedure
Resolution | 59.030 - 1.300 |
R-factor | 0.162 |
Rwork | 0.161 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2og5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.129 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.080 | 0.630 |
Number of reflections | 76258 | |
<I/σ(I)> | 10.1 | 2.6 |
Completeness [%] | 98.3 | 90.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 1.2 M K-/NA-TARTRATE 0.1 M TRIS/HCL PH 8.0 |