2WBN
Crystal structure of the g2p (large terminase) nuclease domain from the bacteriophage SPP1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-08 |
Detector | MARRESEARCH |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.915, 69.915, 72.681 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.520 - 1.900 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22800 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.268 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.430 |
Number of reflections | 16389 | |
<I/σ(I)> | 20 | 3.3 |
Completeness [%] | 99.1 | 96.7 |
Redundancy | 7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 80 MM TRIS PH 8.5, 2.3 M LI2SO4 |