2WBH
Icosahedral particle of covalent coat protein dimer of bacteriophage MS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 368.200, 368.200, 368.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.700 |
| R-factor | 0.318 |
| Rwork | 0.318 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.910 |
| High resolution limit [Å] | 4.700 | 4.700 |
| Rmerge | 0.250 | 1.000 |
| Number of reflections | 72743 | |
| <I/σ(I)> | 2.9 | 0.6 |
| Completeness [%] | 84.0 | 51 |
| Redundancy | 3.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1M BICINE PH 9.0, 20% PEG 5000-MONOETHYL |
