2WB9
Fasciola hepatica sigma class GST
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS ROTATING ANODE GENERATOR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.728, 87.751, 93.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.020 - 1.590 |
| R-factor | 0.17661 |
| Rwork | 0.175 |
| R-free | 0.22243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u3i |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.430 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 62227 | |
| <I/σ(I)> | 6.8 | 4.4 |
| Completeness [%] | 98.2 | 91.9 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15M KBR, 20% PEG 3350 |
