2WB4
activated diguanylate cyclase PleD in complex with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 123.560, 127.430, 88.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.800 |
| R-factor | 0.24053 |
| Rwork | 0.239 |
| R-free | 0.26816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0n |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.599 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.480 | 2.640 |
| High resolution limit [Å] | 2.400 | 2.500 |
| Rmerge | 0.100 | 0.280 |
| Number of reflections | 42374 | |
| <I/σ(I)> | 5.6 | 2.23 |
| Completeness [%] | 87.0 | 62.4 |
| Redundancy | 3.45 | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN SOLUTION\: 100 MICROM PLED, 0.2 MM C-DI-GMP, 2 MM GMPCPP, 1 MM BECL2, 10 MM NAF, 10 MM MGCL2 RESERVOIR\: 0.1 M HEPES, PH 8.0, 0.73 M NA2SO4 |
