2WB2
Drosophila Melanogaster (6-4) Photolyase Bound To double stranded Dna containing a T(6-4)C Photolesion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.550, 89.000, 91.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.950 |
| R-factor | 0.20554 |
| Rwork | 0.204 |
| R-free | 0.24252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cvu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.070 | 0.410 |
| Number of reflections | 15653 | |
| <I/σ(I)> | 13.1 | 3.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PROTEIN WAS MIXED WITH DNA IN A 1:1.1 MOLAR RATIO WITH A FINAL CONCENTRATION OF 8.5MG/ML PROTEIN. 0.1M HEPES PH 7, 15-20% POLYETHYLENGLYCOL 4000, HANGING-DROP VAPOUR DIFFUSION |
