2WB2

Drosophila Melanogaster (6-4) Photolyase Bound To double stranded Dna containing a T(6-4)C Photolesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	87.550, 89.000, 91.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.950
R-factor	0.20554
Rwork	0.204
R-free	0.24252
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvu
RMSD bond length	0.014
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	3.110
High resolution limit [Å]	2.950	2.950
Rmerge	0.070	0.410
Number of reflections	15653
<I/σ(I)>	13.1	3.1
Completeness [%]	99.9	100
Redundancy	5.3	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PROTEIN WAS MIXED WITH DNA IN A 1:1.1 MOLAR RATIO WITH A FINAL CONCENTRATION OF 8.5MG/ML PROTEIN. 0.1M HEPES PH 7, 15-20% POLYETHYLENGLYCOL 4000, HANGING-DROP VAPOUR DIFFUSION