2WAX
Structure of the human DDX6 C-terminal domain in complex with an EDC3- FDF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-06-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.630, 47.850, 65.780 |
| Unit cell angles | 90.00, 96.35, 90.00 |
Refinement procedure
| Resolution | 46.080 - 2.300 |
| R-factor | 0.19319 |
| Rwork | 0.190 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2m |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.728 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.560 |
| Number of reflections | 22482 | |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 98.4 | 99.7 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CAPS PH 10.5, 1.2 M NAH2PO4, 0.8 M K2HPO4, 0.2 M LI2SO4 |
