2WAW
crystal structure of Mycobacterium tuberculosis rv0371c homolog from mycobacterium sp. strain JC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.081, 61.746, 80.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.500 - 1.600 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MYCOBACTERIUM TUBERCULOSIS RV0371C |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 23465 | |
| <I/σ(I)> | 42.8 | 2.8 |
| Completeness [%] | 94.1 | 72.4 |
| Redundancy | 8.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 28% PEG 400, 0.1M HEPES PH 7.5, 0.2M CALCIUM CHLORIDE |
