2WAG
The Structure of a family 25 Glycosyl hydrolase from Bacillus anthracis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 100.522, 100.521, 106.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.730 - 1.400 |
| R-factor | 0.18859 |
| Rwork | 0.187 |
| R-free | 0.21217 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jfx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.614 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | 1.800 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.070 | 0.432 |
| Number of reflections | 62904 | |
| <I/σ(I)> | 6.4 | 20 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 16.3 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 2.1M NH4 SO4, 0.1M TRIS PH 8.5. |
